TALEN™ function in a full-range of animal and plant cells and organisms by evoking highly conserved mechanisms of DNA repair. Recent publications on TAL Effector Nucleases provide an overview on their applications and outline gene knock-out and modification in human cell lines and pluripotent cells (hiPS, hES), rat, mous, zebrafish, yeast, C. elegans, C. briggsae, A.thaliana, N. benthamiana.

TALEN™ can be delivered to cells through standard transfection, nucleoporation and micro-injection approaches as either DNA or mRNA. Our plasmid vector backbones allow expression from CMV or EF1a promoters, whilst the T7 promoter allows for in vitro TALEN™ mRNA synthesis and use.
Our vector backbones enable a full-range of delivery protocols, and we recommend working with a method that ensures highest efficiency delivery in your cells of interest.
For technical support, please contact us.

Yes, TALEN™ have been shown to be able to disrupt multiple copies at once. The efficiency varies on different factors such as cell type, transfection efficiency and TALEN™ activity. We have shown biallelic knock-out in primary human cells at over 50%.

That is dependent on cell lines, transfection conditions, and TALEN™ activity. We recommend testing different ratio of Donor Matrix vs. TALEN™ expression vector. For example, in a gene tagging experiment in U-2-OS cells, we used successfully a ratio of 5µg of Donor Matrix and 10µg of each TALEN™ unit.