How can you achieve efficient targeted integration?
Meganuclease-driven targeted integration is much more efficient than plasmid-derived random integration. Besides, the protocols are designed to optimize the frequency of targeted clone selection and speed.
From the analysis of various transgene models in numerous experiments using the cGPS® and cGPS® Custom CHO-K1 products, more than 90% of the selected clones are targeted.
Can you show evidence of sustained protein production?
We have followed protein expression of lacZ and luciferase from cGPS® CHO-K1 targeted clones over 45 passages. Protein expression was sustained and homogenous over these 22.5 weeks.
cGPS® CHO-K1 protein expression has been shown to be stable overtime even in the absence of antibiotics selection.
Why are clones homogeneous in protein production?
The transgenes are integrated at the same genomic locus and in a single copy. The combination of unique copy integration at a unique genomic locus is necessary to achieve homogeneous protein production within clones.
What types of transgenes have been successfully expressed?
GPCRs, Ca channels, enzymes, mAbs and reporter genes such as lacZ and luciferase have been successfully expressed from cGPS® CHO-K1 targeted cells.
Are GPCRs functional?
Radioligand cell binding, cAMP dosage and cellular dielectric spectroscopy studies performed by users and by us show GPCRs functionality in cGPS® CHO-K1 targeted cells.
Can you show evidence of monoclonal antibody production?
We have successfully expressed from cGPS® CHO-K1 a monoclonal antibody which 2 chains were cloned in the same integration matrix under 2 different promoters. Titer was evaluated in non-optimized 96-well plate growth conditions. (0.7 pg/cell/day)
