Gene Function

Fast and effortless generation of stable and isogenic clones for overexpression and silencing studies

Cellectis bioresearch has developed cGPS^® and cGPS ^® Custom targeted integration kits for the fast and effortless generation of stable and isogenic cell lines from engineered and native cell lines, respectively. You can now easily generate stable and isogenic cell lines overexpressing gene variants and obtain robust functional data for comparison. Stables clones are generated in as few as 19 days and are very stable over time - at least 45 passages - even without selection agents (except for cGPS^® CHO-K1 and cGPS^® Duo CHO-K1 Full Kits).

Your silencing studies can also take advantage of constitutive and targeted expression of shRNAs to easily obtain robust functional data.

All cell lines generated with cGPS^® and cGPS^® Custom Full Kits listed below can be used for Reasearch purposes, see our Sales Terms.

cGPS Custom HepG2 Full Kit

cGPS® (cellular Genome Positioning System) Custom technology based on meganuclease-driven homologous recombination for targeted integration in native cell lines. Enables the effortless generation of stable HepG2 isogenic clones in 36 days. Ideal for robust gene functional studies and data comparisons and for the generation of cell-based assays from laboratory's proprietary derived HepG2 clones. Can be applied to any human derived cell line.

Selection of clones in 36 days
85% of targeted integration events
stable expression >10 weeks even in the absence of selection agents

cGPS Custom MRC-5 Full Kit

cGPS® (cellular Genome Positioning System) Custom technology based on meganuclease-driven homologous recombination for targeted integration in native cell lines. Enables the effortless generation of stable MRC-5 isogenic clones in 27 days. Ideal for robust gene functional studies and data comparisons and for the generation of cell-based assays from laboratory's proprietary derived MRC-5 clones. Can be applied to any human derived cell line.

Selection of clones in 27 days
85% of targeted integration events
stable expression >10 weeks even in the absence of selection agents

cGPS Custom Jurkat Full Kit

cGPS® (cellular Genome Positioning System) Custom technology based on meganuclease-driven homologous recombination for targeted integration in native cell lines. Enables the effortless generation of stable Jurkat isogenic clones in 35 days. Ideal for robust gene functional studies and data comparisons and for the generation of cell-based assays from laboratory's proprietary derived Jurkat clones. Can be applied to any human derived cell line.

Selection of clones in 35 days
85% of targeted integration events
stable expression >10 weeks even in the absence of selection agents

cGPS Custom K-562 Full Kit

cGPS® (cellular Genome Positioning System) Custom technology based on meganuclease-driven homologous recombination for targeted integration in native cell lines. Enables the effortless generation of stable K-562 isogenic clones in 30 days. Ideal for robust gene functional studies and data comparisons and for the generation of cell-based assays from laboratory's proprietary derived K-562 clones. Can be applied to any human derived cell line.

Selection of clones in 30 days
85% of targeted integration events
stable expression >10 weeks even in the absence of selection agents

Gene Function

Gene Function

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