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cGPS

cGPS

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Find your way in pre engineered cell lines

A naturally occurring meganuclease, co-transfected with the GOI (Gene Of Interest) carrying integration matrix, recognizes a pre-integrated sequence in the cell's genome (the meganuclease 'landing pad').

The meganuclease binds to the DNA and induces a DNA double stranded break or 'cut'. Natural repair mechanisms in the cell sense the damage and trigger homologous recombination to enable DNA repair. The co-transfected GOI, flanked by homology regions, is used as a template for the repair. The GOI is stably integrated or 'pasted' at the meganuclease recognition site.



cGPS® Full Kits generate more than 95% of targeted clones after the 2 step selection process. About 80% of the targeted clones are “pure” targeted clones, i.e. only contain a single transgene expression cassette at the targeted genomic site, without any additional randomly integrated copy.

 

Our cGPS® Full Kits contain the necessary ingredients to achieve powerful targeted integration in engineered cell lines:

  • cGPS® Cell Line: modified cell line containing a meganuclease “Landing Pad” stably integrated in a single copy. The Landing Pad is composed of a natural meganuclease recognition site and other genetic elements to support a highly efficient targeted integration two-step selection process,
  • Natural Meganuclease: targets and cleaves a unique recognition site present in an engineered cGPS Cell Line,
  • Integration Matrix: contains a Multiple Cloning Site (MCS) to clone your gene of interest. The MCS is flanked by other genetic elements that are part of the expression cassette (typically promoter and pA signal). The transgene expression cassette is flanked by left and right homology arms. The homology arms are homologous to the genomic regions flanking the meganuclease recognition site and drive homologous recombination and targeted transgene integration at the meganuclease recognition site.

Each individual Full Kit component can be purchased separately from our e-store.

cGPS® engineered Cell Lines are specifically developed on widely used cell lines. Their main advantage resides in their pre-integrated Landing Pad which contains genetic elements to support a highly efficient targeted integration two-step selection process.

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