Cellectis bioresearch offers a full-range of solutions for cell line engineering using proprietary Meganuclease and TALEN™ technologies.

Ready-to-use cGPS® Targeted Integration kits enable the rapid and effortless generation of stable and isogenic cell lines. For biologics manufacturing, a hotspot TI kit based on CHO cells is currently being developed to establish fully-documented cell lines producing biologics, such as monoclonal antibodies in the multiple-gram range, within 6-8 weeks upon transfection. The kit will include amongst others, a CHO-derived cell line bearing a retargetable hotspot, chemically defined medium and optimized protocols for fed-batch production.

Our R&D Services include:

Targeted Integration into a genomic hotspot

Engineered Cell Lines with a retargetable hotspot allow:

• Consistent generation of high-producing cell lines, stable in selection-free medium;
• Screening effort limited to about ten clones to recover mAb-producing cell line in the multi-gram range;
• Development timelines reduced to one month to recover stable clones ready for batch assay;
• Consistent generation of highly-producing pools within two weeks upon transfection to rapidly
• access high quantities of protein of interest.

Cell line optimization through customized gene-specific modifications and knock out.

Meganuclease-induced gene knock-outs allow:

• Enhanced final product quality and efficacy;
• Increased cell doubling time and productivity;
• Reduced downstream processing.

Partnering with us allows our customers to rapidly establish and set up their hotspot retargeting-based platform for cell line development.

Please contact your business development manager for more information.