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Compact TALEN™ are new monomeric modular DNA-binding proteins composed of a single TALE domain fused to the catalytic head of the nuclease I-TevI, designed to cut DNA double-strands in specific locations and enable efficient gene editing.
Monomeric Compact TALEN™ by Cellectis bioresearch
Whereas our Custom TALEN™ require the dimerization of two molecules, this new tool, based on Cellectis’ proprietary TAL effector nuclease technology, makes it possible to target specific DNA segments using a single molecule.
Compact TALEN™ are composed of a TALE domain and the catalytic head of I-TEVI enzyme fused to the N-terminus of the protein. This endonuclease works as a monomer, allowing Compact TALEN™ to work as a single molecule and introduce a DNA double-strand break at a specific target site.
|Product name||Validation level||Cat No.|
|Compact TALEN™||No validation||CTALEN-NV-RCT|
|Compact TALEN™ - Access||Access||CTALEN-ACC-RCT|
|Compact TALEN™ - First human||First human||CTALEN-FRH-RCT|
Benefits of Compact TALEN™:
- Efficient transfection of sensitive cells: Compact TALEN™ enables transfection of sensitive cells (such as primary cells, plant cells, iPS cells [induced pluripotent stem cells] and others) because of its requirement for only a small amount of DNA or RNA (half that needed for a Custom TALEN™ [monomer size ~ 2.7 Kb])
- Ease of viral vectorization: a monomeric Compact TALEN™ makes vectorization easier in viral shuttles
- High specificity: Compact TALEN™ uses a partial DNA-specific endonuclease that recognises the DNA cleavage motif CNNNGN. This results in in vivo DNA cutting capability and specificity that's comparable with Custom TALEN™
To design an optimal Compact TALEN™ a sequence of 2 kb is required. For more information, please contact your local sales manager
Access organisms that are hard to transfect
The transfection of certain organisms or cell types can be difficult, because of the size of the molecules and the quantity of nucleic acid required. This can be a particular problem with certain cell types including primary cells, iPS cells and ES cells (embryonic stem cells). Applications that use viral packaging for delivery can also cause problems – the material can be very expensive, and the size limitations in the viral payload can also make certain experiments impractical. This is where Compact TALEN™ can help.
a) Compact TALEN™ function in tobacco plant protoplasts to induce restoration of a YFP reporter through a single strand annealing assay. TALEN™ were targeted to a sequence fragment of the nptII gene (NPT5::FokI), Compact TALEN™ (TevI::cNPT6L) and negative control (Neg. Ctl). (b) Compact TALEN™ promote targeted gene disruption at endogenous loci in a mammalian cell line. CHO-K1 cells harbouring an integrated neomycin resistance gene were assayed with a TALEN™ (NPT5::FokI), Compact TALEN™ (TevI::cNPT6L) and negative control (Neg. Ctl) targeting NPT5 or cNPT6L, respectively.
The negative control in (a) and (b) does not carry the targeting sequence in the reporter construct.