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Gene knock-out: TALEN™ Sure KO
Highest knock-out success
TALEN™ Sure KO binds its recognition site within the first coding exon, inducing a DNA double-strand break at the recognition site, which is repaired by error prone non homologous end-joining.
How it works
The introduction of frame shift mutations in the start codon region of a gene avoids the production of partially functional truncated proteins and greatly ensures gene knock-out events.
The very high modularity of TALEN™ engineering guarantees that there is always a TALEN™ Sure KO for any gene knock-out you want to achieve.
Find here publications of gene knock-out with TAL Effector Nucleases.
Validation of your choice
TALEN™ Sure KO’s activity can be validated at the level of your choice (no validation, on episomal or chromosomal target sequence). We offer standard validation levels for human, mouse & rat cell lines, as well upon request validate the TALEN™ activity on your cell line. Talk to us about your TALEN™ project and let us define the best TALEN™ for your project.
See below the different validation levels:
First mouse & rat
|definition||optimized TALEN™ design of highest rank, including off-target research||the standard validation for all species other than human||the standard validation for human cell lines||validation on request for mouse & rat cell lines||validation on request for customers' cell lines|
|-||of sequence specific TALEN™ activity on a episomal target||of sequence specific mutagenesis activity on chromosomal target||of sequence specific mutagenesis activity on chromosomal target||of sequence specific mutagenesis activity on chromosomal target|
|-||SSA yeast assay||deep sequencing||deep sequencing||deep sequencing|
|deliverables||2 TALEN™||1 TALEN™ + validation report||2 TALEN™ + validation report||all validated TALEN™ + validation report||all validated TALEN™ + validation report|
|4 weeks||4 weeks**||4 weeks**||9 weeks**||12 weeks**|
*immortalized cell lines
**additional 4 weeks for validation report
Find below a list of our TALEN™ Sure KO options:
|Product name||Validation level||Cat No.|
|TALEN™ Sure KO||No validation||TALEN-SKO-NV-RCT|
|TALEN™ Sure KO - Access||Access||TALEN-SKO-ACC-RCT|
|TALEN™ Sure KO - First human||First human||TALEN-SKO-FRH-RCT|
|TALEN™ Sure KO - First mouse & rat||First mouse & rat||TALEN-SKO-FRMR-RCT|
|TALEN™ Sure KO - Premium||Premium||TALEN-SKO-PRE-RCT|
Specific solutions for methylated DNA and gene modification
Custom TALEN™ Project Support
For any of your gene editing projects, we offer Custom TALEN™ Project Support, a comprehensive service for TALEN™ project optimization. Comes with dedicated project support managers who will share with you competence and know-how to define the best Custom TALEN™ suited to your genome customization project.
Internal data demonstrate that TALEN™ Sure KO efficiently knock-out a GFP transgene that has been stably integrated as a single copy in the CHO-K1 genome, with efficiencies of up to 87% within 6 days post TALEN™ transfection.
>(A) CHO-K1 cell line stably expressing a single copy of the GFP gene (red curve), compared to a GFP negative CHO-K1 control cell line (blue curve) was transiently transfected with GFP specific TALEN™ Sure KO expression vectors; (C) 6 days post transfection, 87% of cells were found GFP negative (red curve); (B) and (D) confocal images corresponding to (A) and (C) respectively.