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TALEN™ Sure KO™
Highest knock-out success
TALEN™ Sure KO™ binds its recognition site within the first coding exon, inducing a DNA double-strand break at the recognition site which is repaired by error prone non homologous end-joining (NHEJ).
The introduction of frame shift mutations in the start codon region of a gene avoids the production of partially functional truncated proteins and greatly ensures gene knock-out events.
The very high modularity of TALEN™ engineering guarantees that there is always a TALEN™ Sure KO™ for any gene knock-out you want to achieve.
Find here publications of gene knock-out with TAL Effector Nucleases.
For licensing enquiries, please contact us.
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Results
In-house results
Internal data demonstrate that TALEN™ Sure KO™ efficiently knock-out a GFP transgene that has been stably integrated as a single copy in the CHO-K1 genome, with efficiencies of up to 60% within 6 days post TALEN™ transfection.
(A) CHO-K1 cell line stably expressing a single copy of the GFP gene (red curve), compared to a GFP negative CHO-K1 control cell line (blue curve) was transiently transfected with GFP specific TALEN™ Sure KO™ expression vectors; (C) 6 days post transfection, 60% of cells were found GFP negative (red curve); (B) and (D) confocal images corresponding to (A) and (C) respectively.
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Specifications
Shipment:Room TemperatureStorage:-20°C
