Culture system: DEF-CS™ 500

Feeder free and defined culture system for efficient expansion and scale up of human pluripotent stem cells

DEF-CS™ 500 is an easy-to-use and robust culture system for the efficient expansion and scale-up of human pluripotent stem cells in a feeder-free and defined environment. The highly reproducible nature of the system coupled with the stable high growth rate makes it ideal for the mass production of cells. The fact that cells are maintained in an undifferentiated state with virtually no background differentiation means that no cell selection is required. Enzymatic passaging as single cells also makes it perfect for single cell applications including high-throughput screening, transfection and seeding onto scaffolds.

DEF-CS™ 500 is a complete culture system including basal medium as well as additives and coating compound. DEF-CS™ 500 is suitable for human embryonic stem cells as well as human induced pluripotent stem cells. A number of human embryonic stem cells cultured in DEF-CS™ 500 are also available for purchase.

• The DEF-CS™ 500 is a complete culture system for both hES cells and hiPS cells;
• You can passage the cells as a single cell suspension for full control of cell seeding;
• This system is robust and allows you to efficiently expand your cells without background differentiation.

Our feeder free medium can be used for a broad range of applications:

• Scale up and mass production of human pluripotent stem cells;
• Bioreactor;
• Transfection and reprogramming;
• Single cell seeding for high throughput screening;
• Seeding in scaffolds (tissue engineering, maturation of human pluripotent stem cells towards tissue like structures).